- Description
- Specifications
Catalog #: CYT-543
Description:
Interferon Alpha Human 2a Recombinant produced in Plant is a single, glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19 kDa.
The Interferon-a 2a contains affinity 6xHis tag on C-terminus.
The IFN-A 2a is purified by proprietary chromatographic techniques.
Synonyms:
Leukocyte interferon, B cell interferon, Type I interferon, IFNA2, IFN-? 2a.
Source:
Nicotiana Sp.Plant.
Amino Acid Sequence:
The sequence of the first twelve N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln-Thr-His-Ser-Leu-Gly-Ser-Arg.
Purity:
Greater than 99.0% as determined by both(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized Interferon-alpha 2a in sterile 18MΩ–cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
Lyophilized, containing 50% SDS.
Stability:
Lyophilized Interferon alpha 2a although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution IFN-alpha 2a should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The specific activity was determined by a quantitative gene report bioassay using human Type I interferon-sensitive cells. Compared with bacterial derived recombinant IFN-a 2a, assayed in the same conditions, plant derived IFN-alpha 2a showed 6.1 x 108 Units/mg in reference to a viral resistance assay using bovine kidney MDBK cells.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
