- Description
- Specifications
Catalog #: CYT-236
Description:
Interferon-beta 1a Human Recombinant produced in CHO (Chinese Hamster Ovarian) cells is a single, glycosylated polypeptide chain containing 166 amino acids and having a molecular mass of 22500 Dalton.
IFN-beta1a is purified by proprietary chromatographic techniques.
Synonyms:
Leukocyte interferon, B cell interferon, Type I interferon, IFNB1, IFB, IFF, IFNB, IFN-b 1a ,MGC96956.
Source:
CHO (Chinese Hamster Ovarian) cells.
Amino Acid Sequence:
MSYNLLGFLQ RSSNFQCQKL LWQLNGRLEY CLKDRMNFDI PEEIKQLQQF QKEDAALTIY EMLQNIFAIF RQDSSSTGWN ETIVENLLAN VYHQINHLKT VLEEKLEKED FTRGKLMSSL HLKRYYGRIL HYLKAKEYSH CAWTIVRVEI LRNFYFINRL TGYLRN.
Purity:
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized Interferon beta 1a in sterile 18MΩ–cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
Lyophilized from 1mg/10ml solution containing 91 mg Human Albumin, and 620 mg mannitol, and 9 mg Sodium Acetate.
Stability:
Lyophilized Interferon-beta 1a although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution IFN-Beta1a should be stored at 4 °C between 2-7 days and for future use below -18 °C.
Please prevent freeze-thaw cycles.
Activity:
The specific activity as determined in a viral resistance assay (human Wish" cell line and VSV virus or the monkey VERO cell line with EMCV virus) was found to be 270 x 106 IU/mg.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
References:
Title:Interferon-_ induces apoptosis in human SH-SY5Y neuroblastoma cells through activation of JAK–STAT signaling and down-regulation of PI3K/Akt pathway.
Publication:Article first published online: 11 NOV 2010 DOI:10.1111/j.1471-4159.2010.07046.x
Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.2010.07046.x/full
