- Description
- Specifications
Catalog #: CYT-358
Description:
Interferon-gamma Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 134 amino acids and having a molecular mass of 15.6kDa.
The IFN-gamma is purified by proprietary chromatographic techniques.
Synonyms:
Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI, IFN-gamma.
Source:
Escherichia Coli.
Amino Acid Sequence:
MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC.
Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized Interferon-gamma in sterile 18M?-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
Lyophilized from a 0.2?m filtered concentrated (1mg/ml) solution in PBS, pH 7.4 and 5% trehalose.
Stability:
Lyophilized Interferon-gamma although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution IFN-gamma should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The specific activity as determined in a viral resistance assay is < 0.1 ng/ml, corresponding to a specific activity of 1 x 107 Units/mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
References:
Title:Interferon-_ and interleukin-4 reciprocally regulate CD8 expression in CD8+ T cells.
Publication:Published online before print November 6, 2008, doi: 10.1073/pnas.0809549105 PNAS November 11, 2008 vol. 105 no. 45 17475-17480
Link:http://www.pnas.org/content/105/45/17475.full
