- Description
- Specifications
Catalog #: CYT-056
Description:
IL-12 p40 human Recombinant produced in Nicotiana benthamiana plant is a glycosilated polypeptide chain containing 312 amino acids, 23-328 aa (molecular formula C1575H2420N424O490S12).
IL-12 p40 is fused to a 6-His-tag at the N-terminal having the total molecular mass of 35.5kDa however as a result of potential glycosylation, the molecular mass could be approximately 40kDa by SDS PAGE under reducing conditions.
IL-12 p40 IS purified by standard chromatographic techniques.
Synonyms:
NKSF2, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor 40 kDa subunit (CLMF p40), TSF, Edodekin-alpha, IL-12 p40, IL-12B, IL-12 subunit p40, NK cell stimulatory factor chain 2.
Source:
Nicotiana benthamiana plant
Amino Acid Sequence:
HHHHHHIWEL KKDVYVVELD WYPDAPGEMV VLTCDTPEED GITWTLDQSS EVLGSGKTLT IQVKEFGDAG QYTCHKGGEV LSHSLLLLHK KEDGIWSTDI LKDQKEPKNK TFLRCEAKNY SGRFTCWWLT TISTDLTFSV KSSRGSSDPQ GVTCGAATLS AERVRGDNKE YEYSVECQED SACPAAEESL PIEVMVDAVH KLKYENYTSS FFIRDIIKPD PPKNLQLKPL KNSRQVEVSW EYPDTWSTPH SYFSLTFCVQ VQGKSKREKK DRVFTDKTSA TVICRKNASI SVRAQDRYYS SSWSEWASVP CS
Purity:
Greater than 97.0% as determined by Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized IL-12 p40 in sterile 18M?-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
Lyophilized from 1mg/ml solution in 20 mM PBS buffer pH 7.4.
Stability:
Lyophilized IL-12 p40 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution IL-12 p40 should be stored at 4 °C between
2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Serological Identification:
The protein was electrophoresed under reducing condition on a 15% SDS-polyacrylamide gel, transferred by electro blotting to a NC membrane and visualized by immune-detection with specific IL12 antibody.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
