- Description
- Specifications
Catalog #: CYT-148
Description:
Interleukin-22 Antagonist Mouse Recombinant produced in E.Coli is a single, non-glycosylated homodimeric polypeptide chain containing 147 amino acids and having a total molecular mass of 16.7 KDa. The Mouse IL-22 Antagonist is purified by proprietary chromatographic techniques.
Synonyms:
IL-TIF, TIFa, IL-10-related T-cell-derived-inducible factor, IL-22, ILTIF, IL-D110, zcyto18, MGC79382, MGC79384, TIFIL-23.
Source:
Escherichia Coli.
Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Leu-Pro-Val-Asn.
Purity:
Greater than 98.0% as determined by:
(a) Gel filtration chromatography under non-denaturing conditions.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Solubility:
It is recommended to reconstitute the lyophilized Interleukin -22 in sterile 18MΩ–cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
IL-22 Antagonist Mouse is lyophilized from a concentrated (1mg/ml) solution in water containing NaHCO3.
Stability:
Lyophilized Interleukin-22 Mouse Antagonist although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution IL22 Antagonist should be stored at 4 °C between 2-7 days and for future use below -18 °C.
Please prevent freeze-thaw cycles.
Activity:
IL-22 E117A mutant is capable of full inhibition of STAT3 phosphorylation induced by mouse interleukin 22 in HepG cells. Its affinity toward immobilized mIL-22 receptor a1 extracellular domain (mIL-22 Ra1-ECD) or IL-22 binding protein is similar to the non-mutated mouse interleukin 22. Mouse IL-22 antagonist (E117A) has very low agonistic activity in this bioassay.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
