- Description
- Specifications
Catalog #: CYT-011
Description:
IL37 (IL1F7) Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 167 amino acids (Lys53-Asp218) and having a molecular mass of 18.6kDa.
The IL-37 (IL-1F7)is purified by proprietary chromatographic techniques.
Synonyms:
Interleukin-37, FIL1 zeta, IL-1X, Interleukin-1 family member 7, IL-1F7, Interleukin-1 homolog 4, IL-1H, IL-1H4, Interleukin-1 zeta, IL-1 zeta, Interleukin-1-related protein, IL-1RP1, Interleukin-23, IL-37, IL37, FIL1Z, IL1F7, IL1H4, IL1RP1, FIL1, FIL1(ZETA).
Source:
Escherichia Coli.
Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Lys-Asn-Leu-Asn.
Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to quick spin followed by reconstitution of IL1F7 in PBS to a concentration no less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
IL1F7 was lyophilized after extensive dialysis against 20mM Phosphate buffer, pH7.4.
Stability:
Lyophilized IL1F7 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution IL1F7 should be stored at 4 °C between 2-7 days and for future use below -18 °C.
Please prevent freeze-thaw cycles.
Activity:
As measured by its binding ability in a functional ELISA, immobilized IL1F7 at 1 µg/ml (100 µl/well) can bind rhIL-18 R/Fc Chimera with a linear range of 0.015-1 µg/ml.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
