- Description
- Specifications
Catalog #: ENZ-073
Description:
Recombinant TOP1 70kDa protein is an enzyme fragment having a molecular mass of 69,199 Dalton (pH 9.4). TOP1 70kDa protein is fused to a hexa-histidine purification tag.
Synonyms:
DNA topoisomerase 1, EC 5.99.1.2, DNA topoisomerase I, TOP1, Scl-70.
Source:
Sf9 insect cells.
Applications:
Western blot with Scleroderma patient sera or monoclonal anti-hexa-His-tag antibody.
Purity:
Greater than 80% as determined by SDS-PAGE.
Formulation:
TOP1 70kDa is supplied in 16mM HEPES buffer pH-8.0, 400mM NaCl, and 20% glycerol.
Coating Concentration:
0.5-0.8 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
Immunological Functions:
Binds IgG-type human auto-antibodies.
Immunological Tests:
Standard ELISA test (checkerboard analysis of positive/negative sera panels, including CDC international reference sera).
Storage:
Store at 4 °C if entire vial will be used within 2-4 weeks. Store, frozen at -20 °C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
