- Description
- Specifications
Catalog #: PKA-220
Description:
JNK2 is a soluble 48 kDa protein that is activated in response to cellular stress, radiation and growth factors. JNK2 binds to the amino terminal activation domains of c-jun or ATF2 regulating AP-1 transcriptional activity.
This protein is the full-length form of the protein with a C-terminal His-tag (His-tag on COOH terminus).
Synonyms:
Mitogen-activated protein kinase 9, EC 2.7.11.24, Stress-activated protein kinase JNK2, c-Jun N-terminal kinase 2, JNK-55, JNK2, JNK2A, JNK2B, PRKM9, JNK2BETA, p54aSAPK, JNK2ALPHA, MAPK9, JUN N-terminal Kinase 2 alpha 1, JNK2a-1.
Source:
Escherichia Coli.
Amino Acid Sequence:
MSDSKCDSQF YSVQVADSTF TVLKRYQQLK PIGSGAQGIV CAAFDTVLGI NVAVKKLSRP FQNQTHAKRAYRELVLLKCV NHKNIISLLN VFTPQKTLEE FQDVYLVMEL MDANLCQVIH MELDHERMSY LLYQMLCGIKHLHSAGIIHR DLKPSNIVVK SDCTLKILDF GLARTACTNF MMTPYVVTRY YRAPEVILGM GYKENVDIWSVGCIMGELVK GCVIFQGTDH IDQWNKVIEQ LGTPSAEFMK KLQPTVRNYV ENRPKYPGIK FEELFPDWIFPSESERDKIK TSQARDLLSK MLVIDPDKRI SVDEALRHPY ITVWYDPAEA EAPPPQIYDA QLEEREHAIEEWKELIYKEV MDWEERSKNG VVKDQPSDAA VSSNATPSQS SSINDISSMS TEQTLASDTD SSLDASTGPLEGCRGGGSHH HHHH.
Purity:
Greater than 85% as determined by SDS-PAGE.
Mass spectrometry of tryptic digest products for identity determination.
Formulation:
25mM HEPES at pH 8.0, 150mM NaCl, 2mM DTT and 10% glycerol.
Physical Appearance:
Sterile Filtered clear solution.
Storage:
Store at 4 °C if entire vial will be used within 2-4 weeks. Store, frozen at -20 °C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Unit Definition:
Activity is approximately 200 units/mg. A certificate of analysis is provided, stating the enzymatic activity for
each production lot. 1.0 unit of activity is defined as the amount of enzyme required to catalyze the transfer of
nmole of phosphate from ATP to to a protein substrate per minute at 30 °C.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
