- Description
- Specifications
Catalog #: ENZ-293
Description:
KLK1 Human Recombinant produced in Yeast is a single, glycosylated, polypeptide chain containing 238 amino acids and having a molecular mass of 28-32 kDa.
KLK1 Human Recombinant is purified by proprietary chromatographic techniques.
Synonyms:
KLK1, KLK-1, HK1, HK-1, KLKR, KLK6, Tissue Kallikrein, hKLK1, EC 3.4.21.35, Kidney/pancreas/salivary gland kallikrein, Kallikrein-1.
Source:
Pichia Pastoris.
Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ile-Val-Gly-Gly-Trp-Glu.
Purity:
Greater than 98.0% as determined by both(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized KLK1 in sterile 18MΩ–cm H2O not less than 100 µg/ml or more than 10 mg/ml solutions.
Formulation:
Lyophilized from a solution containing 1xPBS.
Stability:
Lyophilized KLK1although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution KLK1 should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
Human Kallikrein-1 active Unit Definitin: 1 unit will hydrolize 1 ?mole of H-D-Val-Leu-Arg-pNA(S-2266) per minute at pH 8.0 at 37 °C Human Kallikrein-1 measured biological activity of no less than 5 Units/mg (S2266 method).
Physical Appearance:
Sterile Filtered white lyophilized powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
