- Description
- Specifications
Catalog #: CYT-219
Description:
Keratinocyte Growth Factor-1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 164 amino acids and having a molecular mass of 18995 Dalton.
The FGF-7 is purified by proprietary chromatographic techniques.
Synonyms:
HBGF-7, FGF7, FGF-7, KGF.
Source:
Escherichia Coli.
Amino Acid Sequence:
MCNDMTPEQM ATNVNCSSPE RHTRSYDYME GGDIRVRRLF CRTQWYLRID KRGKVKGTQE MKNNYNIMEI RTVAVGIVAI KGVESEFYLA MNKEGKLYAK KECNEDCNFK ELILENHYNT YASAKWTHNG GEMFVALNQK GIPVRGKKTK KEQKTAHFLP MAIT.
Purity:
Greater than 96.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized Keratinocyte Growth Factor in sterile 18MΩ–cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
Lyophilized from a 0.2?m filtered solution in 20mM PB, pH 8.0, 1M NaCl.
Stability:
Lyophilized Keratinocyte Growth Factor1 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution FGF7 should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The biological activity was determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing KGF receptors yielding an ED50 <10ng/ml, corresponding to a Specific Activity of 1.0x105 IU/mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.9 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of KGF as a Reference Standard.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
References:
1.Title:Human Cord Blood-Derived Endothelial Progenitor Cells and Their Conditioned Media Exhibit Therapeutic Equivalence for Diabetic Wound Healing .
Publication:
Cell Transplantation
ISSN: 0963-6897
DOI: 10.3727/096368910X516637
Volume 19, Issue 12, pages 1635-1644
Copyright © 2010 Cognizant Comm. Corp
Link:http://www.ingentaconnect.com/content/cog/ct/2010/00000019/00000012/art00011
2.Title:Transforming Growth Factor-Alpha: A Major Human Serum Factor that Promotes Human Keratinocyte Migration.
Publication:Journal of Investigative Dermatology (2006) 126, 2096–2105. doi:10.1038/sj.jid.5700350; published online 11 May 2006.
Link:http://www.nature.com/jid/journal/v126/n9/full/5700350a.html
