- Description
- Specifications
Catalog #: CYT-351
Description:
Leptin Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids and having a molecular mass of 16.2 kDa.
The Leptin is purified by proprietary chromatographic techniques.
Synonyms:
OB Protein, Obesity Protein, OBS, Obesity factor.
Source:
Escherichia Coli.
Amino Acid Sequence:
MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSAKQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQVLTSLP SQNVLQIAND LENLRDLLHL LAFSKSCSLP QTSGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDVSPEC.
Purity:
Greater than 95.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized Leptin in sterile 18MΩ–cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
The mouse Leptin was lyophilized from a concentrated (1mg/ml) solution containing 0.1% TFA.
Stability:
Lyophilized Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution Leptin should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
Biological activity of Mouse Leptin is performed in two different mouse obesity models, ob/ob and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5ug Leptin/gram body weight for a period of 14 days. Significant effects on body weight , food consumption, and plasma glucose levels were observed to saline-treated controls.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.201 as the extinction
coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of Leptin as a Reference Standard.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
References:
Title:Severe pulmonary metastasis in obese and diabetic mice.
Publication: Article first published online: 22 SEP 2006 DOI:10.1002/ijc.22248 Copyright © 2006 Wiley-Liss, Inc.
Link:http://onlinelibrary.wiley.com/doi/10.1002/ijc.22248/full
