- Description
- Specifications
Catalog #: CYT-227
Description:
Leptin Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids and having a molecular mass of 16 kDa.
The Leptin is purified by proprietary chromatographic techniques.
Synonyms:
OB Protein, Obesity Protein, OBS, Obesity factor.
Source:
Escherichia Coli.
Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-His.
Purity:
Greater than 95.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized Leptin in sterile 18MΩ–cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
Stability:
Lyophilized Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution Leptin should be stored at 4 °C between 2-7 days and for future use below
-18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
Biological activity of Leptin Rat is evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Protein Content:
Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.201 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of Leptin rat as a Reference Standard.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
References:
1.Title:Expression of Hypothalamic KiSS-1 System and Rescue of Defective Gonadotropic Responses by Kisspeptin in Streptozotocin-Induced Diabetic Male Rats .
Publication: doi: 10.2337/db05-1584 Diabetes September 2006 vol. 55 no. 9 2602-2610
Link:http://diabetes.diabetesjournals.org/content/55/9/2602.full?patientinform-links=yes&legid=diabetes;55/9/2602
2.Title:Desensitization of gonadotropin responses to kisspeptin in the female rat: analyses of LH and FSH secretion at different developmental and metabolic states.
Publication:Am J Physiol Endocrinol Metab 294: E1088–E1096, 2008.294: E1088–E1096, 2008. First published April 15, 2008; doi:10.1152/ajpendo.90240.2008.
Link:http://ajpendo.physiology.org/content/294/6/E1088.full.pdf
3.Title:p38 MAPK and LXR? mediate leptin effect on SREBP-1c expression in hepatic stellate cells (short title: Leptin effect on SREBP-1c expression) .
Publication:
Link:http://www.molmed.org/content/papers%20in%20press/11_243_Yan.pdf
4.Title:Neuroprotection by leptin in a rat model of permanent cerebral ischemia: effects on STAT3 phosphorylation in discrete cells of the brain.
Publication:Citation: Cell Death and Disease (2011) 2, e238; doi:10.1038/cddis.2011.125
Published online 8 December 2011.
Link:http://www.nature.com/cddis/journal/v2/n12/full/cddis2011125a.html
