- Description
- Specifications
Catalog #: CYT-508
Description:
Leptin Binding Domain Human Recombinant also called Leptin soluble receptor produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 208 amino acids and having a molecular mass of 24.5 kDa. Leptin Receptor consists of the cytokine binding domain of leptin receptor amino acids 428-635 of human leptin receptor.
The Leptin Binding Domain is purified by proprietary chromatographic techniques.
Synonyms:
OB Protein, Obesity Protein, OBS, Obesity factor, Leptin Receptor.
Source:
Escherichia Coli.
Amino Acid Sequence:
The sequence of the first four N-terminal amino acids was determined and was found to be Ala-Thr-Pro-Val
Biological ActivityBiological Activity is evidenced by high affinity binding of mammalian leptins at 1:1 molar ratio.
Purity:
Greater than 98.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
The lyophilized Leptin Binding Domain is very soluble in water and most aqueous buffers below and above the isoelectric point.
Formulation:
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
Stability:
Lyophilized Leptin Binding Domain although stable at room temperature, should be stored desiccated below 0 °C. Reconstituted Leptin is best stored refrigerated at 4 °C.
Please prevent freeze-thaw cycles.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Protein Content:
Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 2.45 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of Leptin Binding Domain as a Reference Standard.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
