- Description
- Specifications
Catalog #: ENZ-317
Description:
Matrix Metalloproteinase-13 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 27 kDa.
The Collagenase 3 is purified by proprietary chromatographic techniques.
Synonyms:
Collagenase 3, EC 3.4.24.-, Matrix metalloproteinase-13, MMP-13, CLG3.
Source:
Escherichia Coli.
Applications:
Used as a standard for assaying MMP-13 or for screening inhibitors.
Purity:
Greater than 90% as determined by SDS-PAGE.
Formulation:
The protein Solution (100 µg/ml) in 0.05M Tris-HCl buffer, pH 7.6, containing 0.2M NaCl, 5mM CaCl2, 20 µM ZnSO4 and 0.1% BSA and NaN3.
Stability:
MMP-13 although stable at 4 °C for 1 week, should be stored desiccated below -18 °C.
Please prevent freeze-thaw cycles.
Activity:
Activity is determined by the cleavage of fluorogenic peptide, 100 ng of enzyme activated with APMA will digest 75-80% (1.5-1.6 nmole) of fluorogenic peptide substrate (0.1ml of 20 µM solution) at 35 °C for 30 minutes.
Physical Appearance:
Sterile Filtered clear solution.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
