- Description
- Specifications
Catalog #: ENZ-271
Description:
Matrix Metalloproteinase-7 Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing having a molecular mass of 19.13 kDa.
The MMP-7 is purified by proprietary chromatographic techniques.
Synonyms:
Matrilysin, EC 3.4.24.23, Pump-1 protease, Uterine metalloproteinase, Matrix metalloproteinase-7, MMP-7, Matrin, MPSL1, PUMP-1, MMP7.
Source:
Escherichia Coli.
Purity:
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Formulation:
The protein contains the following additives 10mM HEPES (pH 7.4), 5mM CaCl2 and 150mM NaCl.
Stability:
Matrilysin although stable at 4 °C for 3 weeks, should be stored desiccated below -18 °C.
Please prevent freeze-thaw cycles.
Activity:
The specific activity was found to be 6,900 U/mg.
Physical Appearance:
Sterile clear liquid solution.
Unit Definition:
One unit is defined as the digestion of 1 µg Azocoll/min at 37 °C.
Usage:
Western Blotting (1:1K-5K for neat serum and 1-10 ug/ml for affinity pure antibody using ECL technique).
ELISA: Control peptide can be used to coat ELISA plates at 1 ug/ml and detected with antibodies (1:10-50K for neat serum and 0.5-1 ug/ml for affinity pure).
Histochemistry & Immunofluorescence: Not tested.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
