- Description
- Specifications
Catalog #: ENZ-285
Description:
Thyroid Peroxidase Human Recombinant produced in SF9 is a glycosylated, polypeptide chain containing 834 amino acids and having a molecular mass of 92,872 Dalton (excluding glycosylation), 101 kDa total mass.
The TPO is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
Synonyms:
Thyroid peroxidase, EC 1.11.1.8, TPO, MSA, TPX.
Source:
Sf9 insect cells.
Applications:
Western-Blot with monoclonal anti-TPO antibody and monoclonal anti-hexa-His-tag antibody.
Purity:
Greater than 95% as determined by SDS-PAGE.
Formulation:
TPO is supplied in 16mM HEPES pH-7.6, 160mM NaCl, 0.08mM Kl and 20% glycerol.
Coating Concentration:
0.15-0.375 µg/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination.
Immunological Functions:
1. Binds IgG-type human auto-antibodies.
2. Auto-antibodies to TPO recognize conformation-dependent epitopes.
3. Standard ELISA test (checker-board analysis of positive/negative sera panels, including international reference serum NIBSC 66/387).
Storage:
Store at 4 °C if entire vial will be used within 2-4 weeks.
Store, frozen at -20 °C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
