- Description
- Specifications
Catalog #: CYT-568
Description:
PDGF-AA Human Recombinant produced in Yeast is a homodimeric, glycosilated, polypeptide chain containing 2 x 110 amino acids and having a total molecular mass of 34 kDa.
PDGF-AA is purified by proprietary chromatographic techniques.
Synonyms:
Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-AA, PDGF-1.
Source:
Pichia Pastoris.
Purity:
Greater than 98.0% as determined by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized PDGF-AA in sterile 18MΩ–cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
The protein was lyophilized with 20mM sodium phosphate buffer.
Stability:
Lyophilized PDGF-AA although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution PDGF-AA should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The ED50 was found to be 1ng/ml corresponding to a Specific Activity of 1,000,000IU/mg calculated by the ability to stimulate the proliferation of mouse 3T3 fibroblasts (PNAS 94, 10205, 1997. Biochemistry, 1996, 35, 12077).
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
