- Description
- Specifications
Catalog #: ENZ-275
Description:
The 29 kDa Aeromonas Aminopeptidase is produced by genetic engineering and can be used for physical & structural investigations, sequence and amino-terminal determinations. This exopeptidase recognizes a specific stop sign at –X- Pro and requires a free a-amino group in the L-configuration. It is therefore suitable for the removal of the redundant N-terminal methionine often added to engineered recombinant proteins.
Synonyms:
Bacterial leucyl aminopeptidase, EC 3.4.11.10.
Source:
Aeromonas Proteolytica.
Purity:
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Formulation:
Buffered solution containing 10mM Tris-HCl, 100mM NaCl and 5 µM ZnSO4, pH 8.0.
Stability:
Two years when stored at -20 °C, 2 weeks at 4 °C.
Activity:
Recombinant Aeromonas Aminopeptidase was found to have an activity of 120 Units/mg protein.
Physical Appearance:
Sterile filtered liquid formulation.
Unit Definition:
One unit is defined as the amount of enzyme that hydrolyses 1 µmole of L-leucine p-nitroanilide at 25 °C per 1 minute.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
