- Description
- Specifications
Catalog #: ENZ-375
Description:
DPPIV Human Recombinant produced in High-5 cells is a single, glycosylated polypeptide chain containing 746 amino acids (39-766) and having a molecular mass of 86.4 kDa.
DPPIV is fused to His Tag at C-terminus and purified using conventional chromatography techniques.
Synonyms:
CD26, ADABP, ADCP2, DPPIV, TP103, DPP4, Dipeptidyl peptidase 4, Dipeptidyl peptidase IV, DPP IV, T-cell activation antigen CD26, Adenosine deaminase complexing protein 2, CD26 antigen.
Source:
High-5 cells.
Amino Acid Sequence:
ADP-SRKTYTLTDYLKNTYRLKLYSLRWISDHEYLYKQENN
ILVFNAEYGNSSVFLENSTFDEFGHSINDYSISPDGQ
FILLEYNYVKQWRHSYTASYDIYDLNKRQLITEERIP
NNTQWVTWSPVGHKLAYVWNNDIYVKIEPNLPSYRIT
WTGKEDIIYNGITDWVYEEEVFSAYSALWWSPNGTFL
AYAQFNDTEVPLIEYSFYSDESLQYPKTVRVPYPKAG
AVNPTVKFFVVNTDSLSSVTNATSIQITAPASMLIGD
HYLCDVTWATQERISLQWLRRIQNYSVMDICDYDESS
GRWNCLVARQHIEMSTTGWVGRFRPSEPHFTLDGNSF
YKIISNEEGYRHICYFQIDKKDCTFITKGTWEVIGIE
ALTSDYLYYISNEYKGMPGGRNLYKIQLSDYTKVTCL
SCELNPERCQYYSVSFSKEAKYYQLRCSGPGLPLYTL
HSSVNDKGLRVLEDNSALDKMLQNVQMPSKKLDFIIL
NETKFWYQMILPPHFDKSKKYPLLLDVYAGPCSQKAD
TVFRLNWATYLASTENIIVASFDGRGSGYQGDKIMHA
INRRLGTFEVEDQIEAARQFSKMGFVDNKRIAIWGWS
YGGYVTSMVLGSGSGVFKCGIAVAPVSRWEYYDSVYT
ERYMGLPTPEDNLDHYRNSTVMSRAENFKQVEYLLIH
GTADDNVHFQQSAQISKALVDVGVDFQAMWYTDEDHG
IASSTAHQHIYTHMSHFIKQCFSLP-SGRLVPRGSHHHHHH.
Purity:
Greater than 95.0% as determined by Analysis by SDS-PAGE.
On SDS-PAGE under denatured condition, apparent molecular weight of glycosylated DPP4 will migrate at approximately 90kDa.
Formulation:
DPP4 is formulated in 20mM Tris-HCl buffer pH-8, 100mM NaCl, 1mM EDTA and 10% glycerol.
Stability:
Store at 4 °C if entire vial will be used within 2-4 weeks. Store, frozen at -20 °C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Activity:
>20 Units/mg.
Physical Appearance:
Sterile Filtered colorless solution.
Assay Methods:
- Reaction buffer: 20mM Tris pH8.0, 0.1M NaCl, 1mM EDTA
- Total reaction volume: 100 ul
- Reaction temperature: 37 °C. 1. Add the reaction buffer to each well.
2. Add the 10 ul of 10mM substrate (Gly-Pro p-nitroanilide) to each well.
3. Add the enzyme (DPP-4) diluent to each well.
4. Incubate the 96 well plate at 37 °C.
5. Read the optical density at 405 nm. Optical density was measured at 405 nm after incubating enzyme solution with 1mM of p-nitroanilid as a substrate. 0ng DPP4= 0 OD at 405nm.
20ng DPP4= 0.075 OD at 405nm.
40ng DPP4= 0.125 OD at 405nm.
60ng DPP4= 0.2 OD at 405nm.
80ng DPP4= 0.275 OD at 405nm.
100ng DPP4= 0.35 OD at 405nm.
Unit Definition:
One unit will hydrolyze 1 umole of p-nitroaniline per minute at pH8.0 at 37 °C using 1mM of Gly-Pro p-nitroanilde as a substrate.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
