- Description
- Specifications
Catalog #: ENZ-348
Description:
Uridine phosphorylase from Salmonella typhimurium (StUP) catalyzes the reversible phosphorolysis of uridine with the formation of ribose-1-phosphate and uracil.
Description:
Uridine phosphorylase Salmonella typhimurium Recombinantproduced in E.Coli is a non-glycosylated, polypeptide having a total molecular mass of 163068 Dalton.
Synonyms:
Uridine phosphorylase, EC 2.4.2.3, UrdPase, UPase, StUP.
Source:
Escherichia Coli.
Purity:
Greater than 95.0% as determined by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized UPase in sterile 18MΩ–cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
The UPase was lyophilized from 1mg/ml solution containing 25mM Tris-HCl, pH 8.0, 0.15M NaCl.
Stability:
Lyophilized UPase although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution UPase should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Enzyme Activity:
30 U/mg protein.
Physical Appearance:
Sterile Filtered white lyophilized powder.
Activity Determination:
One unit phosphorylates 1µm of uridine within 1 min at pH 7.3.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
