- Description
- Specifications
Catalog #: PRO-646
Description:
The recombinant Protein A/G consists of 7 IgG-binding domains EDABC-C2C3, which corresponds to the Protein A and G domains that are included in the recombinant sequence. The Protein A portion is from Staphylococcus aureus segments E, D, A, B and C. The Protein G portion is from Streptococcus segments C2 and C3. The fusion protein has a predicted molecular mass of 50.5 kDa. But it migrates with an apparent molecular mass of approximately 45kDa in SDS-PAGE.
Source:
Escherichia coli.
Applications:
Protein A/G is an excellent tool for purification and detection of mouse monclonal antibodies from IgG subclasses without interference from these other serum proteins. Individual subclasses of mouse monoclonals are most likely to have stronger affinity to this chimeric protein than to either Protein A or Protein G. Binding is less pH-dependent than either Protein A or Protein G alone, occurring well at pH 5-8.
Purity:
>95% as determined by SDS-PAGE and RP-HPLC.
Formulation:
Lyophilized white Powder containing no additives.
Reconstitution:
Reconstitution with deionized water or PBS.
Storage:
After reconstitution, aliquot and store at -20 °C.
Avoid repeated freeze/thaw cycles.
A280 of 0.1% Solution:
~0.505.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
