- Description
- Specifications
Catalog #: PKA-324
Description:
PKC-q Human Recombinant produced in Sf9 is a glycosylated, polypeptide chain containing amino acids 2-706 and having a molecular mass of 86 KD. This protein is the full-length form of the protein with an amino terminal poly His-tag.
PKC-q is purified by proprietary chromatographic techniques.
Synonyms:
Protein kinase C theta type, EC 2.7.11.13, nPKC-theta, PRKCQ, PRKCT, MGC126514, MGC141919.
Source:
Sf9 insect cells.
Purity:
Greater than 85% as determined by SDS-PAGE.
Formulation:
PKC-q is supplied at a of 0.1mg/ml in 10mM Tris, pH 7.4, 0.1M NaCl, 20% glycerol, 1mM DTT, 0.1mM EDTA, 0.2mM PMSF and 0.03% Brij-35.
Physical Appearance:
Sterile Filtered clear solution.
Storage:
Store at 4 °C if entire vial will be used within 2-4 weeks.
Store, frozen at -20 °C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Unit Definition:
~ 1800 units/mg. One unit of PKCq activity transfers 1 nmole of phosphate to a PKCe pseudosubstrate peptide per minute at 30 °C using. Recombinant human active PKCq also phosphorylates histone, MARCKS, moesin, RasGRP3, and PKCa and PKCe pseudosubstrates. PKCq is a novel PKC and does not require calcium for activity. Activity may vary depending on the substrate and reaction conditions. The optimal concentration should be determined for each specific application. PKCq is involved in T-cell activation and T-cell receptor signaling including T-cell mediated activation of NF-kB and AP-1.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH
USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
