- Description
- Specifications
Catalog #: HOR-266
Description:
Parathyroid Hormone Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 28 amino acids and having a molecular mass of 3380 Dalton.
The PTH is purified by proprietary chromatographic techniques.
Synonyms:
PTH/PTHrP receptor antagonist, PTHrP analog, PTHR, MGC138426, MGC138452.
Source:
Escherichia Coli.
Amino Acid Sequence:
Leu-Met-His-Asn-Leu-Gly-Lys-His-Leu-Asn-Ser-Met-Glu-Arg-Val-Glu-Trp-Leu-Arg-Lys-Lys-Leu-Gln-Asp-Val-His-Asn-Phe.
Purity:
Greater than 97.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized PTH in sterile 18MΩ–cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
The protein (1 mg/ml) was lyophilized after extensive dialyses against 20mM PBS pH-7.0 and 4% mannitol.
Stability:
Lyophilized Parathyrin although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution PTH should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The activity calculated by UMR106 cell/cAMP method corresponding to a specific activity of 10,000 Units/mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
