- Description
- Specifications
Catalog #: CYT-334
Description:
sRANKL Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 176 amino acids and having a molecular mass of 20006 Dalton.
CD254 is purified by proprietary chromatographic techniques.
Synonyms:
Soluble Receptor Activator of NFkB Ligand, TNFSF11, TRANCE, TNF-related activation-induced cytokine, OPGL, ODF, Osteoclast differentiation factor, Tumor necrosis factor ligand superfamily member 11, Receptor activator of nuclear factor kappa B ligand, RANKL, Osteoprotegerin ligand, CD254 antigen, sRANKL, sOdf, hRANKL2.
Source:
Escherichia Coli.
Amino Acid Sequence:
MEKAMVDGSW LDLAKRSKLE AQPFAHLTIN ATDIPSGSHK VSLSSWYHDR GWAKISNMTF SNGKLIVNQD GFYYLYANIC FRHHETSGDL ATEYLQLMVY VTKTSIKIPS SHTLMKGGST KYWSGNSEFH FYSINVGGFF KLRSGEEISI EVSNPSLLDP DQDATYFGAF KVRDID.
Purity:
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized sRANKL in sterile 18MΩ–cm H2O at a concentration of 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
The protein was lyophilized from a concentrated (1mg/ml) solution containing 10mM Na2PO4, pH-8.0.
Stability:
Lyophilized TNFSF11 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution sRANKL should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The activity as determined by its ability to induce a dose dependant stimulation of IL-8 production in human PBMCs, is 39-51ng/ml.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
References:
Title:Cathepsin K Expression and Activity in Canine Osteosarcoma.
Publication: Article first published online: 15 DEC 2011 DOI:10.1111/j.1939-1676.2011.00834.x Copyright © 2011 by the American College of Veterinary Internal Medicine.
Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1939-1676.2011.00834.x/full
