- Description
- Specifications
Catalog #: CYT-453
Description:
RELM-alpha Mouse Recombinant is manufactured with a signal sequence of phage fd (20aa) and C-terminal fusion of flagTag (10aa). The RELM-alpha Flag-Tagged Fusion Protein is a 13.3 kDa protein containing 91 amino acid residues with 30 additional amino acid residues - signal sequence of phage fd, flagTag (underlined).
MKKLLFAIPL VVPFYSHSTM VNTDETIEII VENKVKELLA NPANYPSTVT TLSCTSVKT MNRWASCPAG MTATGCACGF ACGSWEIQSG DTCNCLCLLV DWTTARCCQL SLEDYKDDDD K.
Synonyms:
Resistin-like alpha, RELMalpha, Cysteine-rich secreted protein FIZZ1, Parasite-induced macrophage novel gene 1 protein, Cysteine-rich secreted protein A12-gamma, RELM-a.
Source:
Escherichia Coli.
Applications:
Western blotting, ELISA.
Purification Method:
Two-step procedure using size exclusion chromatography before and after refolding.
Purity:
Greater than 95% as determined by SDS-PAGE.
Solubility:
Add 0.2 ml of deionized H2O and let the lyophilized pellet dissolve completely.
Formulation:
Sterile filtered and lyophilized from 0.5 mg/ml in 5mM Tris pH 7.5, 25mM NaCl.
Stability:
Store lyophilized protein at -20 °C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 °C for a limited period of time; it does not show any change after two weeks at 4 °C.
Specificity:
The amino acid sequence of the RELM-alpha Mouse recombinant is 100% homologous to the amino acid sequence of the RELM-alpha Mouse.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
