- Description
- Specifications
Catalog #: PRO-459
Description:
SMAD4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 572 amino acids (1-552) and having a molecular mass of 62.6 kDa. SMAD4 is fused to a 20 amino acid His-Tag at N-Terminus and purified by standard chromatography techniques.
Synonyms:
JIP, DPC4, MADH4, SMAD-4, DPC-4, MADH-4, Mothers against decapentaplegic homolog 4, Mothers against DPP homolog 4, SMAD 4, hSMAD4, Deletion target in pancreatic carcinoma 4, SMAD4, SMAD family member 4.
Source:
Escherichia Coli.
Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MDNMSITNTP TSNDACLSIV HSLMCHRQGG ESETFAKRAI ESLVKKLKEK KDELDSLITA ITTNGAHPSK CVTIQRTLDG RLQVAGRKGF PHVIYARLWR WPDLHKNELK HVKYCQYAFD LKCDSVCVNP YHYERVVSPG IDLSGLTLQS NAPSSMMVKD EYVHDFEGQP SLSTEGHSIQ TIQHPPSNRA STETYSTPAL LAPSESNATS TANFPNIPVA STSQPASILG GSHSEGLLQI ASGPQPGQQQ NGFTGQPATY HHNSTTTWTG SRTAPYTPNL PHHQNGHLQH HPPMPPHPGH YWPVHNELAF QPPISNHPAP EYWCSIAYFE MDVQVGETFK VPSSCPIVTV DGYVDPSGGD RFCLGQLSNV HRTEAIERAR LHIGKGVQLE CKGEGDVWVR CLSDHAVFVQ SYYLDREAGR APGDAVHKIY PSAYIKVFDL RQCHRQMQQQ AATAQAAAAA QAAAVAGNIP GPGSVGGIAP AISLSAAAGI GVDDLRRLCI LRMSFVKGWG PDYPRQSIKE TPCWIEIHLH RALQLLDEVL HTMPIADPQP LD.
Purity:
Greater than 90.0% as determined by SDS-PAGE.
Formulation:
The SMAD4 protein solution contains 20mM Tris-HCl pH-8, and 20% glycerol.
Stability:
Store at 4 °C if entire vial will be used within 2-4 weeks. Store, frozen at -20 °C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Physical Appearance:
Sterile Filtered colorless solution.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
References:
Title:Transforming Growth Factor _-Induced Reactivation of Epstein-Barr Virus Involves Multiple Smad-Binding Elements Cooperatively Activating Expression of the Latent-Lytic Switch BZLF1 Gene .
Publication:Published ahead of print 18 May 2011, doi: 10.1128/_JVI.01197-10 J. Virol. August 2011 vol. 85 no. 15 7836-7848
Link:http://jvi.asm.org/content/85/15/7836.full
