- Description
- Specifications
Catalog #: PRO-283
Description:
The Streptavidin preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native streptavidin. Streptavidin has a molecular weight of 55kDa.
Source:
Bacterium Streptomyces avidinii.
Applications:
Streptavidin may be used to visualize biotin conjugated molecules in ELISA, blotting and histological technique.
Purity:
Greater than 95.0% as determined by SDS-PAGE.
Solubility:
Gives a clear solution at 5mg/ml in 0.1M NaCl.
Formulation:
Lyophilized (1mg/ml) in 50mM NaCl, pH 9.0.
Stability:
Streptavidin although stable at 4 °C for 3 weeks, should be stored desiccated below -18 °C.
For longer storage in dissolved form add 1mM EDTA and/or 0.02 % NaN3 or pass the solution through a sterile filter.
Please prevent freeze-thaw cycles.
Proteolytic Activity:
Less than 10-3 U/mg protein (Azocoll, 25 °C, 24 h, pH 8.0).
Specific Activity:
Greater than 15.9 U/mg, 1 unit binds 1 µg biotin.
Physical Appearance:
Sterile Filtered lyophilized powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
