- Description
- Specifications
Catalog #: PRO-208
Description:
SODA produced in E.Coli is a single, non-glycosylated polypeptide chain containing 226 amino acids (1-206 a.a.) and having a molecular mass of 25.2kDa.
SODA is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Synonyms:
Superoxide dismutase [Mn], MnSOD, soda, b3908, JW3879.
Source:
Escherichia Coli.
Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MSYTLPSLPY AYDALEPHFD KQTMEIHHTK HHQTYVNNAN AALESLPEFA NLPVEELITK LDQLPADKKT VLRNNAGGHA NHSLFWKGLK KGTTLQGDLK AAIERDFGSV DNFKAEFEKA AASRFGSGWA WLVLKGDKLA VVSTANQDSP LMGEAISGAS
GFPIMGLDVW EHAYYLKFQN RRPDYIKEFW NVVNWDEAAA RFAAKK.
Purity:
Greater than 95% as determined by SDS-PAGE.
Formulation:
SODA E.coli solution (1mg/ml) containining 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% glycerol and 0.1M NaCl.
Stability:
SODA E.Coli Recombinant although stable at 4 °C for 1 week, should be stored below -18 °C.
Please prevent freeze thaw cycles.
Activity:
Specific activity is > 350 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and xanthine oxidase at pH 7.8 at 25C in a 1.5 ml reaction volume.
Physical Appearance:
Sterile filtered colorless solution.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
