- Description
- Specifications
Catalog #: ENZ-280
Description:
Alanine Aminotransferase Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 495a.a and having a molecular mass of 54,479 Dalton. The amino acid sequence is the same as that of native form of human liver ALT.
The ALT is purified by proprietary chromatographic techniques.
Synonyms:
ALT1, Glutamic-pyruvic transaminase 1, GPT 1, Glutamic-alanine transaminase 1, AAT1, ALT, ALAT, SGPT.
Source:
Escherichia Coli.
Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Formulation:
The protein was dialyzed against 40mM sodium acetate buffer (pH 5.5), 1mM DTT,1mM EDTA, 5mM 2-oxoglutarate and 0.1mM pyridoxal-5'-phosphate.
Stability:
AAT1 although stable at 10 °C for 5 days, should be stored below -18 °C.
Please prevent freeze-thaw cycles.
Activity:
The specific activity was found to be 1,020 U/mg.
Physical Appearance:
Sterile liquid formulation.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
