- Description
- Specifications
Catalog #: CYT-561
Description:
Human Transforming Growth Factor-beta 1 purified from Human Platelets having a molecular mass of 25kDa.
The TGF-b 1 is purified by proprietary chromatographic techniques.
Synonyms:
Transforming growth factor beta-1, TGF-beta-1, CED, DPD1, TGFB, TGF-b 1.
Source:
Human Platelets.
Purity:
Greater than 97.0%.
Solubility:
It is recommended to reconstitute lyophilized TGF-beta 1 in sterile 100 µl 5mM HCl, which can then be further diluted to the desired aliquot to 1ml of PBS containing 2mg/ml Albumin.
Formulation:
TGF-Beta1 protein was lyophilized from a solution containing 5mM Sodium Citrate (200 µl) pH 3.5.
Stability:
Lyophilized TGF-beta 1 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution TGF-beta 1 should be stored at 4 °C between 2-7 days and for future use below -18 °C.
DO NOT RECONSTITE WITH NEUTRAL BUFFERS.
DO NOT USE GLASS IMPLEMENTS OR EXTENSIVE MANIPULATIONS.
PREVENT FREEZE THAW CYCLES.
Activity:
Stimulates the growth of NRK-1 cells in soft agar at concentrations ranging from 0.1 to 5ng/ml corresponding to a specific activity of 200,000-10,000,000IU/mg. Effective concentration ranges must be experimentally determined. Purified EGF and/or TGF-alpha at concentrations of at least 2ng/ml must be present for observation of the biological activity.
Physical Appearance:
Sterile Filtered lyophilized powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
