- Description
- Specifications
Catalog #: PRO-770
Description:
Recombinant Human Trypsin-2 is free from any animal and human sources.
Recombinant Human Trypsin-2 expressed in E.Coli having a Mw of 24kDa is purified by standard chromatography techniques.
Recombinant Human Trypsin-2 is free from foreign enzymes such as carboxypeptidase A & chymotrypsin. Recombinant Human Trypsin-2 is free from protease inhibitors such as PMSF and EDTA.
Source:
Escherichia Coli.
Purity:
Greater than 90% as determined by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized Human Trypsin in sterile 18MΩ–cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
The protein was lyophilized without 10mM Sodium Acetate buffer and 50mM NaCl.
Stability:
Recombinant Human Trypsin although stable at room temp for 1 week, should be stored desiccated below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Activity:
10,000 BAEE units/mg powder.
Physical Appearance:
Sterile Filtered lyophilized powder.
Unit Definition:
One BAEE unit will produce a Delta A253 of 0.001 per minute in a reaction volume of 3.0ml at pH7.6 at 25C (1cm light path). Trypsin digestion: suggested ratio (w:w) 1:20 to 1:1000 (Trypsin : Peptide).
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
