- Description
- Specifications
Catalog #: CYT-223
Description:
Tumor Necrosis Factor-a Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 158 amino acids (157 a.a. of the mature human TNF-alpha and an N-terminal methionine) and having a molecular mass of 17.5kDa.
The TNF-alpha is purified by standard chromatographic techniques.
Synonyms:
TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.
Source:
Escherichia Coli.
Amino Acid Sequence:
MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA E AKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL.
Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile 18MΩ–cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
1mg of TNF-alpha Human contain 20mM PB, pH-7.2, and 100mM NaCl.
Stability:
Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution TNF-a should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The Specific Activity is >5.0x107 IU/mg as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Protein Content:
Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 1.234 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of TNF-a as a Reference Standard.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
References:
1.Title: Radiation-triggered Tumor Necrosis Factor (TNF) ?-NF?B Cross-signaling Favors Survival Advantage in Human Neuroblastoma Cells*
Publication:First Published on April 28, 2011, doi: 10.1074/jbc.M110.193755 June 17, 2011 The Journal of Biological Chemistry, 286, 21588-21600.
Link:http://www.jbc.org/content/286/24/21588
2.Title:Bidirectional Concentration-Dependent Effects of Tumor Necrosis Factor Alpha in Shigella dysenteriae-Related Seizures.
Publication:INFECTION AND IMMUNITY, Apr. 2003, p. 2288–2291 Vol. 71, No. 40019-9567/03/$08.000 DOI: 10.1128/IAI.71.4.2288–2291.2003Copyright © 2003, American Society for Microbiology. All Rights Reserved.
Link:http://iai.asm.org/content/71/4/2288.full.pdf
3.Title:Common Inhibitory Serine Sites Phosphorylated by IRS-1 Kinases, Triggered by Insulin and Inducers of Insulin Resistance.
Publication:First Published on April 19, 2007, doi: 10.1074/jbc.M610949200 June 22, 2007 The Journal of Biological Chemistry, 282, 18018-18027.
Link:http://www.jbc.org/content/282/25/18018.full
4.Title:Antioxidative function of L-FABP in L-FABP stably transfected Chang liver cells.
Publication:Article first published online: 20 SEP 2005 DOI:10.1002/hep.20857 Copyright © 2005 American Association for the Study of Liver Diseases.
Link:http://onlinelibrary.wiley.com/doi/10.1002/hep.20857/full
5.Title:Interferon-? induces apoptosis in human SH-SY5Y neuroblastoma cells through activation of JAK–STAT signaling and down-regulation of PI3K/Akt pathway
Publication:Article first published online: 11 NOV 2010 DOI:10.1111/j.1471-4159.2010.07046.x © 2010 The Authors. Journal of Neurochemistry © 2010 International Society for Neurochemistry.
Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.2010.07046.x/full
6.Title:Common Inhibitory Serine Sites Phosphorylated by IRS-1 Kinases, Triggered by Insulin and Inducers of Insulin Resistance.
Link:http://journal.9med.net/qikan/article.php?id=408762
7.Title:The human respiratory syncytial virus non-structural protein 1 regulates type I and type II interferon pathways.
Publication:First Published on February 8, 2012, doi: 10.1074/mcp.M111.015909 mcp.M111.015909.
Link:http://www.mcponline.org/content/early/2012/02/08/mcp.M111.015909.short
8.Title:Calcium transient evoked by TRPV1 activators is enhanced by tumor necrosis factor-? in rat pulmonary sensory neurons.
Publication:Published online before print July 16, 2010, doi: 10.?1152/?ajplung.?00111.?2010 AJP - Lung Physiol October 1, 2010 vol. 299 no. 4 L483-L492
Link:http://ajplung.physiology.org/content/299/4/L483.full
