- Description
- Specifications
Catalog #: CYT-116
Description:
Recombinant Human VEGF 121 amino acids, produced in HEK cells is a glycosylated 37kDa homodimer and 50kDa homotrimer.
The VEGF is purified by proprietary chromatographic techniques.
Synonyms:
Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.
Source:
HEK.
Purity:
Greater than 95% as obsereved by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized VEGF in sterile water not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
The VEGF was lyophilized from 1mg/ml in 1xPBS.
Stability:
Lyophilized VEGF although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution VEGF should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The specific activity was determined by the dose-dependent stimulation of the proliferation of HUVEC cells (Human Umbilical Vein Endothelial Cells) and is typically 2-8ng/ml.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
