- Description
- Specifications
Catalog #: CYT-619
Description:
Vascular Endothelial Growth Factor-121 Human Recombinant produced in E.Coli is a double, non-glycosylated, polypeptide chain containing a total of 142 amino acids and having a molecular mass of 16.3 kDa. The VEGF-121 is fused to 20 amino acid His tag at N-terminus and purified by proprietary chromatographic techniques.
Synonyms:
Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.
Source:
Escherichia Coli.
Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQEKCDKP RR.
Purity:
Greater than 95.0% as determined by SDS-PAGE.
Formulation:
VEGF-121 His Tag in 20mM TRIS pH-8.
Stability:
Liquid VEGF 121 although stable at 10 °C for 1 week, should be stored below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The ED50 for this effect is <4.2 ng/ml. Measured in a cell proliferation assay using NIH-3T3 cell, corresponding to a specific activity of less than 238,095.23units/mg.
Physical Appearance:
Sterile Filtered colorless solution.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
