- Description
- Specifications
Catalog #: PRO-309
Description:
Vimentin Human Recombinant is produced in E.Coli, having a molecular weight of 53,685 Dalton (calculated from sequence), 57,000 Dalton (determined by SDS gelelctrophoresis).
Synonyms:
Vimentin, Vim, FLJ36605.
Source:
Escherichia Coli.
Amino Acid Sequence:
mstrsvssss yrrmfggpgt asrpsssrsy vttstrtysl gsalrpstsr slyasspggv yatrssavrl rssvpgvrll qdsvdfslad aintefkntr tnekvelqel ndrfanyidk vrfleqqnki llaeleqlkg qgksrlgdly eeemrelrrq vdqltndkar veverdnlae dimrlreklq eemlqreeae ntlqsfrqdv dnaslarldl erkveslqee iaflkklhee eiqelqaqiq eqhvqidvdv skpdltaalr dvrqqyesva aknlqeaeew ykskfadlse aanrnndalr qakqesteyr rqvqsltcev dalkgtnesl erqmremeen faveaanyqd tigrlqdeiq nmkeemarhl reyqdllnvk maldieiaty rkllegeesr islplpnfss lnlretnlds lplvdthskr tlliktvetr dgqvinetsq hhddle.
Purity:
Greater than 95.0% as determined by SDS-gelelctrophoresis.
Solubility:
It is recommended to reconstitute the Vimentin in sterile 18MΩ–cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
The protein (1mg/ml) was lyophilized with 30mM Tris/HCL, pH 8, 9.5M urea, 2mM EDTA, 2mM DTT 10mM methylammonium chloride.
Stability:
Lyophilized Vimentin although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution Vimentin should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Reconstitution to Filaments:
After vimentin is dissolved in 9.5M urea buffer (see above), protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50mM NaCl, 2mM dithiothreitol, 10mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
- Hatzfeld M. and Franke W.W. (1985). J. Cell Biol. 101, 1826-1841
- Hatzfeld M. et al. (1987). J. Mol. Biol. 197, 237-255.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
